Application

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CFDP1 in 10 µg of Jurkat cell lysate.

Research CategoryNeuroscience

Anti-CFDP1, clone 15E5.1, Cat. No. MABN396, is a highly specific mouse monoclonal antibody that targets CFDP1 and has been tested for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Craniofacial development protein 1 (UniProt: Q9UEE9; also known as Bucentaur) is encoded by the CFDP1 (also known as BCNT, CENP-29) gene (Gene ID: 10428) in human. Craniofacial development protein 1 is an evolutionarily conserved member of the Bucentaur (BCNT) family of proteins and displays ubiquitous expression. It contains a BCNT domain of about 80 amino acids in the C-terminal region. Two isoforms of craniofacial development protein 1 are reported that are generated by alternative splicing. It is reported to play a role in embryogenesis and is considered as essential for chromatin/chromosome organization and function. A unique feature of this protein is that it lacks cysteine residues and has an acidic N-terminal region and a lysine/glutamic acid/proline-rich region of 40 amino acids. It also has seven serine phosphorylation sites and Ser250 in the BCNT region is shown to be heavily phosphorylated. (Ref.: Messina, G., et al. (2015). Chromosoma 124(2):153 162; Iwashita, S et al. (2015). Biosci Rep. 35(4): e00228).

Immunogen

GST-taged recombinant fragment corresponding to the first 111 amino acids from the N-terminal region.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CFDP1 in 10 µg of HEK293 cell lysate.

Specificity

Clone 15E5.1 detects Craniofacial development protein 1 in human. It targets an epitope within the first 111 amino acids from the N-terminal half.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~45 kDa observed; 33.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).